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m ml334  (MedChemExpress)


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    Structured Review

    MedChemExpress m ml334
    Mechanisms underlying the effects of HELLS on autophagy-dependent ferroptosis. (A) Co-immunoprecipitation was performed in HK-1 cells with either HELLS antibody or normal rabbit IgG antibody, followed by immunoblot analysis using the indicated antibodies. (B) Relative viability of the sh-HELLS, sh-NC, sh-HELLS + <t>ML334</t> and sh-NC + ML334 groups at 0, 24, 48 and 72 h. Levels of (C) GSH, MDA and (D) Fe 2+ in the four groups. (E-G) Relative mRNA expression levels of (E) GPX4, (F) HO-1 and (G) Nrf2 in the four groups. (H) Representative images of protein blots of GPX4, HO-1 and Nrf2. (I) Relative protein expression levels of GPX4 HO-1 and Nrf2 in the four groups. (J) Representative images of protein blots of Beclin1and LC3. (K) Relative protein expression levels of Beclin1and LC3 in the four groups. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. HELLS, lymphoid-specific helicase; sh-, short hairpin; NC, negative control; GSH, glutathione; MDA, malondialdehyde; GPX4, glutathione peroxidase 4; HO-1, heme oxygenase-1; Nrf2, nuclear factor-erythroid 2-related factor 2.
    M Ml334, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m ml334/product/MedChemExpress
    Average 94 stars, based on 8 article reviews
    m ml334 - by Bioz Stars, 2026-06
    94/100 stars

    Images

    1) Product Images from "HELLS inhibits autophagy-dependent ferroptosis in nasopharyngeal carcinoma by modulating the Nrf2/HO-1/GPX4 pathway"

    Article Title: HELLS inhibits autophagy-dependent ferroptosis in nasopharyngeal carcinoma by modulating the Nrf2/HO-1/GPX4 pathway

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2026.5788

    Mechanisms underlying the effects of HELLS on autophagy-dependent ferroptosis. (A) Co-immunoprecipitation was performed in HK-1 cells with either HELLS antibody or normal rabbit IgG antibody, followed by immunoblot analysis using the indicated antibodies. (B) Relative viability of the sh-HELLS, sh-NC, sh-HELLS + ML334 and sh-NC + ML334 groups at 0, 24, 48 and 72 h. Levels of (C) GSH, MDA and (D) Fe 2+ in the four groups. (E-G) Relative mRNA expression levels of (E) GPX4, (F) HO-1 and (G) Nrf2 in the four groups. (H) Representative images of protein blots of GPX4, HO-1 and Nrf2. (I) Relative protein expression levels of GPX4 HO-1 and Nrf2 in the four groups. (J) Representative images of protein blots of Beclin1and LC3. (K) Relative protein expression levels of Beclin1and LC3 in the four groups. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. HELLS, lymphoid-specific helicase; sh-, short hairpin; NC, negative control; GSH, glutathione; MDA, malondialdehyde; GPX4, glutathione peroxidase 4; HO-1, heme oxygenase-1; Nrf2, nuclear factor-erythroid 2-related factor 2.
    Figure Legend Snippet: Mechanisms underlying the effects of HELLS on autophagy-dependent ferroptosis. (A) Co-immunoprecipitation was performed in HK-1 cells with either HELLS antibody or normal rabbit IgG antibody, followed by immunoblot analysis using the indicated antibodies. (B) Relative viability of the sh-HELLS, sh-NC, sh-HELLS + ML334 and sh-NC + ML334 groups at 0, 24, 48 and 72 h. Levels of (C) GSH, MDA and (D) Fe 2+ in the four groups. (E-G) Relative mRNA expression levels of (E) GPX4, (F) HO-1 and (G) Nrf2 in the four groups. (H) Representative images of protein blots of GPX4, HO-1 and Nrf2. (I) Relative protein expression levels of GPX4 HO-1 and Nrf2 in the four groups. (J) Representative images of protein blots of Beclin1and LC3. (K) Relative protein expression levels of Beclin1and LC3 in the four groups. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. HELLS, lymphoid-specific helicase; sh-, short hairpin; NC, negative control; GSH, glutathione; MDA, malondialdehyde; GPX4, glutathione peroxidase 4; HO-1, heme oxygenase-1; Nrf2, nuclear factor-erythroid 2-related factor 2.

    Techniques Used: Immunoprecipitation, Western Blot, Expressing, Negative Control



    Similar Products

    m ml334  (MedChemExpress)
    94
    MedChemExpress m ml334
    Mechanisms underlying the effects of HELLS on autophagy-dependent ferroptosis. (A) Co-immunoprecipitation was performed in HK-1 cells with either HELLS antibody or normal rabbit IgG antibody, followed by immunoblot analysis using the indicated antibodies. (B) Relative viability of the sh-HELLS, sh-NC, sh-HELLS + <t>ML334</t> and sh-NC + ML334 groups at 0, 24, 48 and 72 h. Levels of (C) GSH, MDA and (D) Fe 2+ in the four groups. (E-G) Relative mRNA expression levels of (E) GPX4, (F) HO-1 and (G) Nrf2 in the four groups. (H) Representative images of protein blots of GPX4, HO-1 and Nrf2. (I) Relative protein expression levels of GPX4 HO-1 and Nrf2 in the four groups. (J) Representative images of protein blots of Beclin1and LC3. (K) Relative protein expression levels of Beclin1and LC3 in the four groups. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. HELLS, lymphoid-specific helicase; sh-, short hairpin; NC, negative control; GSH, glutathione; MDA, malondialdehyde; GPX4, glutathione peroxidase 4; HO-1, heme oxygenase-1; Nrf2, nuclear factor-erythroid 2-related factor 2.
    M Ml334, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m ml334/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    m ml334 - by Bioz Stars, 2026-06
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Mechanisms underlying the effects of HELLS on autophagy-dependent ferroptosis. (A) Co-immunoprecipitation was performed in HK-1 cells with either HELLS antibody or normal rabbit IgG antibody, followed by immunoblot analysis using the indicated antibodies. (B) Relative viability of the sh-HELLS, sh-NC, sh-HELLS + ML334 and sh-NC + ML334 groups at 0, 24, 48 and 72 h. Levels of (C) GSH, MDA and (D) Fe 2+ in the four groups. (E-G) Relative mRNA expression levels of (E) GPX4, (F) HO-1 and (G) Nrf2 in the four groups. (H) Representative images of protein blots of GPX4, HO-1 and Nrf2. (I) Relative protein expression levels of GPX4 HO-1 and Nrf2 in the four groups. (J) Representative images of protein blots of Beclin1and LC3. (K) Relative protein expression levels of Beclin1and LC3 in the four groups. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. HELLS, lymphoid-specific helicase; sh-, short hairpin; NC, negative control; GSH, glutathione; MDA, malondialdehyde; GPX4, glutathione peroxidase 4; HO-1, heme oxygenase-1; Nrf2, nuclear factor-erythroid 2-related factor 2.

    Journal: International Journal of Molecular Medicine

    Article Title: HELLS inhibits autophagy-dependent ferroptosis in nasopharyngeal carcinoma by modulating the Nrf2/HO-1/GPX4 pathway

    doi: 10.3892/ijmm.2026.5788

    Figure Lengend Snippet: Mechanisms underlying the effects of HELLS on autophagy-dependent ferroptosis. (A) Co-immunoprecipitation was performed in HK-1 cells with either HELLS antibody or normal rabbit IgG antibody, followed by immunoblot analysis using the indicated antibodies. (B) Relative viability of the sh-HELLS, sh-NC, sh-HELLS + ML334 and sh-NC + ML334 groups at 0, 24, 48 and 72 h. Levels of (C) GSH, MDA and (D) Fe 2+ in the four groups. (E-G) Relative mRNA expression levels of (E) GPX4, (F) HO-1 and (G) Nrf2 in the four groups. (H) Representative images of protein blots of GPX4, HO-1 and Nrf2. (I) Relative protein expression levels of GPX4 HO-1 and Nrf2 in the four groups. (J) Representative images of protein blots of Beclin1and LC3. (K) Relative protein expression levels of Beclin1and LC3 in the four groups. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. HELLS, lymphoid-specific helicase; sh-, short hairpin; NC, negative control; GSH, glutathione; MDA, malondialdehyde; GPX4, glutathione peroxidase 4; HO-1, heme oxygenase-1; Nrf2, nuclear factor-erythroid 2-related factor 2.

    Article Snippet: To investigate whether autophagy-dependent ferroptosis was regulated through the Nrf2/HO-1/GPX4 signaling pathway, cells were initially stimulated with erastin and subsequently treated with 1 μ M ML334 (cat. no. HY-110258; MedChemExpress) for 24 h.

    Techniques: Immunoprecipitation, Western Blot, Expressing, Negative Control